Also known as comparative modelling of protein, is the method of creating an atomic resolution model of target protein from amino acid sequence. This is considered as one of the accurate technique to determine computational protein structure. The model functionality is dependent on the quality of the structure generated, increasing the quality of homology modelling is important. First step in this process is identification of homologous sequence in the structure database to use as templates for modelling. Followed by this, alignment of target and template sequence is done. A framework is built, which is the structure of target protein consisting main chain atoms. Addition and optimisation of side chain atoms and loops is the fourth step. Refine and optimise the entire model and finally evaluate the overall quality. Uses are analysis of protein function, their interactions and antigenic behaviour as well as in drug development. More information about the topic can be understood from the PowerPoint presentation.

Shivangi Ojha

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