The process entails introducing a foreign fragment of DNA into the genome containing the desired gene. This particular gene that is introduced is referred to as the recombinant gene and the technique is known as the recombinant DNA technology. DNA ligase, Reverse transcriptase, Restriction endonuclease, Terminal transcriptase, Nuclease, DNA polymerase, Ribonuclease-H, Alkaline phosphatase, Polynucleotide kinase are the principally used enzymes for recombinant DNA technology. Tools of Recombinant DNA technology. The enzymes which include the restriction enzymes help to cut, the polymerases- help to synthesize and the ligases- help to bind. The restriction enzymes used in recombinant DNA technology play a major role in determining the location at which the desired gene is inserted into the vector genome. The first step in Recombinant DNA technology is to isolate the desired DNA in its pure form and then cutting the gene at the recognition sites.

Hima Xavier

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