Recombinant DNA technology alters the phenotype of an organism (host) through a genetically altered vector. This cloning vector is introduced and integrated into the genome of the organism. Thus the process involves the introduction of a foreign piece of DNA into the genome which contains our gene of interest. The gene which is introduced is the recombinant gene and the technique is called the recombinant DNA technology. Recombinant DNA technology can be complete and achieved with the help of some elemental tools. The different tools used for the purpose are restriction enzymes, vectors and host organism. The restriction enzymes used in recombinant DNA technology play a major role in determining the location at which the desired gene is inserted into the vector genome. The vectors form a very important part of the tools of recombinant DNA technology as they are the ultimate vehicles that carry forward the desired gene into the host organism. The host is the ultimate tool of recombinant DNA technology which takes in the vector engineered with the desired DNA with the help of the enzymes.

Preena N

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