Antisense DNA technology is a method to inhibit or downregulate the production of a target protein by using antisense DNA or RNA molecules. Antisense and RNAi are both synthesized in the lab and delivered to patients to decrease the expression of a disease-associated protein. RNA interference (RNAi) is the biological process of mRNA degradation induced by complementary sequences double-stranded small interfering RNAs (siRNA's) and suppression of target gene expression. Exogenous siRNAs play an important role in host defense against RNA viruses and in transcriptional and post-transcriptional gene regulation in plants and other eukaryotes. Using RNAi technology by transfecting synthetic siRNAs into eukaryotic cells to silence genes has become an indispensable tool to investigate gene functions, and siRNA-based therapy is being developed to knockdown genes implicated in diseases. Have a look at the resourceful presentation.

Ridhi Kumari

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